电针对自发性高血压大鼠的血压昼夜节律以及下丘脑和心脏RORα、BMAL1表达的影响

Effects of Electroacupuncture on Circadian Rhythm of Blood Pressure and Expression of RORα and BMAL1 in Hypothalamus and Heart of Spontaneously Hypertensive Rats

    摘要
  • 摘要:
    目的 观察电针对自发性高血压大鼠（Spontaneously hypertensive rat，SHR）血压昼夜节律以及心脏和下丘脑RORα、BMAL1表达的影响，探讨电针调节血压昼夜节律紊乱的可能机制。
    方法 选取20只16周龄SHR，适应性饲养一周后，将20只SHR随机分为模型组、电针组、假电针组、西药组，另选同周龄5只Wistar Kyoto大鼠（WKY）作为对照组，选取双侧内关穴、双侧足三里穴、百会穴进行针刺干预。测量干预前后大鼠的24 h动态血压和体质量，以每日8：00动物房开灯时间规定ZT0，每4 h测量一次血压。用免疫组化、免疫荧光、Western blot观察大鼠心脏、下丘脑RORα、BMAL1蛋白表达；用实时荧光定量PCR（qRT-PCR）检测大鼠心脏及下丘脑RORα、BMAL1的转录水平。
    结果 与模型组相比，电针组血压昼夜节律振荡存在昼低夜高的节律特点，杓型率升高（P<0.001），心脏及下丘脑RORα、BMAL1蛋白表达以及转录水平升高（P<0.05，P<0.001）；假电针组大鼠血压未能达到正常昼夜节律振荡水平，心脏及下丘脑RORα、BMAL1表达无明显差异；西药组血压夜节律振荡水平较模型组好转，但杓型率未见明显差异，心脏及下丘脑中RORα蛋白的表达升高（P<0.001），下丘脑BMAL1蛋白及转录水平均明显升高（P<0.01，P<0.001）。
    结论 电针可以改善高血压大鼠血压昼夜节律紊乱，其作用机制可能是通过上调心脏及下丘脑RORα、BMAL1的表达以调节血压昼夜节律。

     

    Abstract:
    OBJECTIVE To observe the impacts of electroacupuncture on the circadian rhythm of blood pressure, the protein and transcription expression of RORα and BMAL1 of the heart and hypothalamus of spontaneously hypertensive rats, and explore the mechanism of electroacupuncture in treating disturbance of circadian rhythm of blood pressure.
    METHODS Twenty 16-week-old spontaneously hypertensive rats were selected and acclimatized for one week before being randomly assigned to four groups: model group, electroacupuncture group, sham electroacupuncture group, and western medicine group. Additionally, 5 age-matched Wistar-Kyoto (WKY) rats were selected as a control group. Acupuncture intervention was administered at the bilateral "Neiguan" (PC6) and "Zusanli" (ST36) acupoints, as well as the "Baihui" (GV20) acupoint. The 24-hour ambulatory blood pressure and body weight of rats were measured before and after the intervention; blood pressure was recorded every 4 hours, with ZT0 defined as 08:00 (the time the animal facility lights were turned on). Immunohistochemistry, immunofluorescence, and Western blot were used to observe the protein expression of RORα and BMAL1 in the heart and hypothalamus, while quantitative real-time PCR (qRT-PCR) was used to detect the transcriptional levels of RORα and BMAL1 in these tissues.
    RESULTS Compared with the model group, the blood pressure in the electroacupuncture group was low in light and high at dark, the dipper ratio was increased (P<0.001), and the expression of RORα and BMAL1 in the heart and hypothalamus were all increased (P<0.05,P<0.001,). In the sham electroacupuncture group, the blood pressure could not reach the normal level of circadian oscillation and there were no significant differences in the expression of RORα and BMAL1 in the heart and hypothalamus. In the western medicine group, the level of nocturnal rhythmic oscillations of blood pressure was better than that in the model group, but no significant difference was seen in the dipper rate, the expression of RORα protein in the heart and hypothalamus were both elevated (P<0.001), and the hypothalamus BMAL1 protein and transcription levels were significantly elevated (P<0.01,P<0.001).
    CONCLUSION Electroacupuncture can improve the circadian rhythm disorder of blood pressure in hypertensive rats, which may be related to regulate the circadian rhythm system by up-regulating the expression of RORα and BMAL1 in the heart and hypothalamus.

     

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